I met someone, who had LIT with another doctor in the past. They got shocked to see my 16 giant mosquito bites. She said she just had 2 mosquito bites with her paternal lymphocyte immunization.
With the protocol of our doctor, four donor at a time. Each donors are extracted with 70 mL blood. After processing, the lymphocyte was just below 1/3 mL. We did intradermal injection, 4x per donor.
The other doctor's protocol, 500 ml were extracted from her husband. She said the lymphocytes were injected on the skin also. I guess the lymphocyte collected was about 2~3ml. I guess they did subcutaneous injection because they had two giant mosquito bites only.
One more difference is the CMV cytomegalovirus seronegativity issue. My first immunologist doesn't care about CMV. My current immunologist only allows CMV- donor for CMV- recipient.
What is efficacy of intradermal vs subcutaneous LIT? I am really curious why she had implantation failure. Next time, I will ask her Th1/Th2 ratio and other immune issues.
http://humrep.oxfordjournals.org/content/21/2/429.full
http://www.transfusion.com.au/blood_products/components/modified_blood/cmv_seronegative
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