Does stress contribute to Infertility?

Am I infertile because I am stressed?
Am I stressed because I am infertile?
Does cortisol level has something to do with infertility?
Can stress trigger NK cells?
Does cortisol trigger NK cells, T-cells, T helper1...?
In the past generation, APAS (anti-phospholipid antibody syndrome) is never heard. Why nowadays it is prevalent?

Our karyotype result

What a relief! Normal 46,xx and 46,xy. Thank God.

My delayed cytotoxic reaction on LIT

Delayed cytoxic reaction and delayed hypersentivity reaction is the same.  It takes 2 to 3 days to develop. This explains why my LIT sites started to reduce its swelling and rebounded back after the third day.

This reminds me of my tetanus skin test. I did not react to the skin test within 15 minutes. My rashes developed few hours after the intramuscular injection. What is delayed type hypersensitivity mean on my previous tetanus toxoid skin test? I never had tetanus antibody, therefore no antibody to react with the tetanus toxoid yet? Or I have other immunologic factor?  I will ask again this issue to my immunologist.

I remembered he mentioned about th1/th2 balance. My Th1 is high. Hmmm... Haha next time I propose pro-histamine to induce th2 pathway. high th1 + high th2 = balanced th1/th2 ^.^ What if my wicked proposal is valid? Yeah, I dont have to worry about tnf-alpha... Tnf-alpha will be counted by il-10. Haha I can proceed with IVF,  no need for another round of LIT ^.^  just kidding...

Poor me, my obgyne asked how many sessions of LIT do I need? I replied I don't know. If I heard it right, my immunologist said in the past, I need four sessions of LIT. But my last consultation, he said he needs to evaluate again after my next LIT. Delayed cytotoxic reaction involves th1- it is problematic for him.

https://www.boundless.com/microbiology/immunology-applications/immunity-disorders-hypersensitivity/type-iv-delayed-cell-mediated-reactions/
"Type IV hypersensitivity is often called delayed type hypersensitivity as the reaction takes two to three days to develop. Unlike the other types, it is not antibody mediated but rather is a type of cell-mediated response. CD4+ helper T cells recognize antigen in a complex with Class 2 major histocompatibility complex. The antigen-presenting cells in this case are macrophages that secrete IL-12, which stimulates the proliferation of further CD4+ Th1 cells. CD4+ T cells secrete IL-2 and interferon gamma, further inducing the release of other Th1 cytokines, thus mediating the immune response. Activated CD8+ T cells destroy target cells on contact, whereas activated macrophages produce hydrolytic enzymes and, on presentation with certain intracellular pathogens, transform into multinucleated giant cells."

LIT injection site and CMV issue

I met someone, who had LIT with another doctor in the past. They got shocked to see my 16 giant mosquito bites. She said she just had 2 mosquito bites with her paternal lymphocyte immunization.

With the protocol of our doctor, four donor at a time. Each donors are extracted with 70 mL blood. After processing, the lymphocyte was just below 1/3 mL. We did intradermal injection, 4x per donor.

The other doctor's protocol, 500 ml were extracted from her husband. She said the lymphocytes were injected on the skin also. I guess the lymphocyte collected was about 2~3ml. I guess they did subcutaneous injection because they had two giant mosquito bites only.

One more difference is the CMV cytomegalovirus seronegativity issue. My first immunologist doesn't care about CMV. My current immunologist only allows CMV- donor for CMV- recipient.

What is efficacy of intradermal vs subcutaneous LIT? I am really curious why she had implantation failure.  Next time, I will ask her Th1/Th2 ratio and other immune issues.

http://humrep.oxfordjournals.org/content/21/2/429.full
http://www.transfusion.com.au/blood_products/components/modified_blood/cmv_seronegative

TH1/TH2 - the untested new theory

http://www.autoimmunemom.com/immune-system/autoimmunity-th1-th2-cells.html
"The medical community is divided over whether achieving this balance is really a key part of restoring and maintaining health in the face of autoimmune disease."

"It is thought that an overactive TH1 system needs to be balanced by stimulating the TH2 pathway, and vice versa. Since the former is involved mainly in cellular infections, one approach involves increasing TH2 levels via deliberate allergic stimuli.  Conversely, down-regulating the TH2 response might possibly involve intentional infection (as with vaccines) with microbes in order to increase TH1 response and therefore restore balance."

Wahaha, sounds logical and interesting. I will ask my immunologist about this!
I have experienced uveitis, that was triggered by tetanus toxoid vaccine. Doctor said overproduction of T-cell, th1 pathway... Who knows,

- beer is the cure for uveitis attack.
- half bottle of red wine before IUI.
- two shot of Tequila before IVF.

I am allergic to alcoholic beverage- a possible trigger for my Th2 pathway. Simple problem gets simple solution.

My current supplements: calcitrol, fish oil, L-glutathione, L-carnitine, coQ10
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http://www.altmedrev.com/publications/8/3/223.pdf
http://autoimmune-paleo.com/what-is-the-role-of-th1-and-th2-in-autoimmune-disease/
http://www.rue309.com/book/autoimmune_disorders.shtml
http://www.precisionnutrition.com/rr-green-tea-hazards

My first LIT - reaction and interpretation

My consultation with immunologist was cancelled last week.  Today is 10th day after my LIT. Luckily, I photographed the first 5 days after my LIT.

1. My forearm is wrapped with bandage for four days to protect from UV light. UV light weakens the lymphocyte reaction.

2. The pustules are dead lymphocytes. The strong reaction means I am strongly capable of stimulating my immune cells. Good news, I did not react much to my husband's lymphocyte. 

3. Normally in two days, the inflammation should start to subside.  And it should continue to subside until the inflammation is out.

4. In my case, I had a delayed cytotoxic hypersensitivity reaction. 

After my macrophage has detected the foreign lymphocyte and engulfed it.  Macrophages have broken down these substances and present the smaller proteins to the T lymphocytes.  Then, my t-cells duplicate and attack the dead foreign lymphocyte again. There was also subcutaneous blood leak and swollen peripheral tissues. I thought because I did not have a good rest. Doctor said my T helper cell 1 is not balanced. My Th1/Th2 ratio is high.

 5. After learning that tetanus toxoid has triggered uveitis, I was afraid that LIT may cause uveitis. I complained I had tight eye muscle. Luckily, no uveitis. Doctor said LIT should alleviate the autoimmune disease because we are working to lower the Th1/Th2 ratio.

Polyps < 1cm

Small polyps (polyps <1 cm) may regress on their own. OBGYNE1 said she has a patient, who had large polyps. The patient got pregnant before the scheduled polypectomy. There is no need to remove my polyp. Am I spotting between periods? My left fallopian tube is not blocked. My main problems are immunology and husband's sperm count.

She feels I am so stubborn. I showed her my LIT marks to prove that I am following her advice.